Apparatus (1 M) for sterilisation of a female mammal comprising at least one separation member (307) for separation of a fallopian tube (15) from a uterine cavity (17) of the female mammal, a circulation assembly which is operatively connected to said at least one separation member (307), and at least one sterilisation lumen (4b) which is operatively connected to said circulation assembly and debouching at a distal side of said at least one separation member (307), said at least one sterilisation lumen (4b) being adapted for injection in use of a tissue necrotising fluid capable of necrotising a lining of at least a part of said fallopian tube (15), wherein said circulation assembly is adapted to circulate said tissue necrotising fluid within said sterilisation lumen (4b) and said fallopian tube (15). The apparatus (1 M) allows quick, simple, safe and reliable sterilisation.