KANEKA CORPORATION;NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY;HYOGO COLLEGE OF MEDICINE;FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION;NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER
An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysis and/or dispase； and filtering the enzyme-treated amnion through a mesh； a method for producing a cryopreserved mesenchymal stromal cell composition； and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.
