A 4,6-hexadecadiene-2,4-dicarboxylic acid derivative represented by the following general formula (I) or a pharmaceutically acceptable salt thereof, each of which has an anti-tumor activity, and the like, are provided. [wherein, R 1 represents hydroxy, -OR 3 (wherein R 3 represents optionally substituted lower alkyl or an optionally substituted aliphatic heterocyclic group), or - NR 4 R 5 (wherein R 4 and R 5 may be the same or different, and each represents a hydrogen atom or optionally substituted lower alkyl) and R 2 represents hydroxy or -OR 6 (wherein R 6 represents optionally substituted lower alkyl or optionally substituted aralkyl)]
