The present invention belongs to the technical field of biomedicine. Provided are an improved anti-VEGFR-2 monoclonal antibody and an application thereof. By a computer-aided simulation design, a novel phage antibody library was designed, and an improved anti-VEGFR-2 monoclonal antibody was obtained after multiple rounds of screening. Both affinity and biological activity of said antibody are higher than the original antibody. Said antibody can effectively inhibit the binding of VEGFR-2 and a ligand thereof, VEGF, in vitro and can be used in treating a tumor and a disease caused by angiogenesis such as macular degeneration.
