A pet grooming assembly includes a brush that has a handle portion and a bristle portion. The bristle portion has a chamber within the bristle portion. The handle portion has a reservoir within the handle portion wherein the reservoir may contain a liquid medication. The chamber and the reservoir are in fluid communication with each other. A valve is movably attached to the brush and the valve selectively inhibits a flow of the liquid medication from the reservoir to the chamber. A plurality of first bristles is attached to the bristle portion wherein each of the first bristles may brush an animal. A plurality of second bristles is attached to the bristle portion. Each of the second bristles is substantially hollow such that each of the second bristles is in fluid communication with the chamber. Thus, each of the second bristles may release the liquid medication onto the animal.