In some embodiments, imaging apparatus for imaging blood vessels within a target region of tissue includes a housing having an aperture which, in use, is placed against a target region of tissue such that the target region of tissue occludes the aperture, a light source arranged to illuminate at least a portion of a target region of tissue occluding the aperture and to provide illuminating light having predetermined first and second spectral ranges which are different, an imaging device arranged to receive illuminating light reflected by the target region of tissue occluding the aperture, wherein the imaging device is configured to generate image output at the first and second spectral ranges, and a controlling element arranged to selectively control the imaging device and/or the light source to sequentially capture at least one image at the first spectral range and at least one image at the second spectral range.
