Jan Johansson,Gäran Hjäm,Margareta Stark,Anna Rising,Stefan Grip,Wilhelm Engsträm,My Hedhammar
申请号：
US14086244
公开号：
US08729235B2
申请日:
2013.11.21
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.
