A growth regulator specially for Jatropha curcas L. is made by the following method: dissolving cytokinins in sodium hydroxide solution, then diluting with distilled water to mother solution with cytokinins concentration of 0.02mg/mL- 100mg/mL, uniformly mixing 2-9999 parts mother solution, 1-10 parts surfactant and 0-9997 parts distilled water to obtain said growth regulator. In 10 days before or after appearance of Jatropha curcas L. flower buds, uniformly spraying said growth regulator onto whole plant of Jatropha curcas L. until droplet drips down. After spraying 1-3 times during each Jatropha curcas L. blossoming period, total flowers, female proportion, fruits and seed yield are 3.6, 4.3, 4.5 and 3.9 times higher than untreated respectively. Oil content of the seed is increased to 34.76% from 31.67% and increased by about 9.8%. The present growth regulator operates simply and has lower cost.