1 1-1 O GC N 1-1 00 1-1 O N C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 16 August 2018 (16.08.2018) WIPO I PCT olimion °noI0 DIDmoIIVmoIIIII DIIIIII DID (10) International Publication Number WO 2018/147801 Al DO: Seed and culture hPSCs on OP9 cells (51) International Patent Classification: C12N 5/0783 (2010.01) A61K 35/17 (2015.01) C12N 5/10 (2006.01) A61P 35/00 (2006.01) (21) International Application Number: PCT/SG2018/050051 (22) International Filing Date: 07 February 2018 (07.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201700937P 07 February 2017 (07.02.2017) SG 10201705582S 06 July 2017 (06.07.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]； 1 Fusionopolis Way #20-10 Connexis, Singapore 138632 (SG). (72) Inventors: ZENG, Jieming； c/o Institute of Bioengineer- ing and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). WANG, Shu； c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). (74) Agent: YUSARN AUDREY； 24 Raffles Place #27-01 Clif- ford Centre, Singapore 048621 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, G
