Kouta Hatayama,Yoshiharu Asaoka,Toru Tanaka,Teruhiko Ide
申请号：
US13583550
公开号：
US09453066B2
申请日:
2011.03.10
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Disclosed are: an Fc binding protein having increased stability with respect to heat, acid, and/or alkalinity compared with the wild type a method for producing same and a method for specifically isolating protein containing an Fc binding protein binding site using said Fc binding protein as a ligand for affinity chromatography.An Fc binding protein was obtained having increased stability with respect to heat, acid, and/or alkalinity compared with the wild-type human Fc receptor by means of substituting at least one specific amino acid residue in the extracellular domain of the wild-type human Fc receptor with another amino acid residue. The Fc binding protein is useful as a ligand for affinity chromatography for example by immobilizing in a solid phase. Also, when the Fc binding protein is expressed using a host that has been transformed with an expression vector containing a polynucleotide coding for said protein, the amount of produced protein is increased compared with using a wild-type human Fc receptor.
