Stimulation assembly in support of inseminating livestock, particularly in inseminating a sow, comprising a bow provided with a first flank part, a second flank part and a back part keeping the flank parts substantially at a fixed distance from each other, wherein the flank parts and the back part define an accommodation space inside of the bow for accommodating livestock inside it, wherein the back part and the flank parts in placed condition of the stimulation assembly on the livestock are situated opposite the back and flanks, respectively, of the livestock, wherein on the side of the bow facing the accommodation space the stimulation assembly is provided with an air cushion that is adapted for being coupled to an external air source, wherein in a first position the air cushion takes up a first volume inside of the bow and in a second position it takes up a second volume inside of the bow that exceeds the first volume.
