Various embodiments of the invention relate to polypeptides comprising 1-10 or more epitopes of the HIV envelope protein and a fusion protein, wherein the polypeptide lacks the transmembrane domain of the HIV gp41 protein. Such polypeptides may be expressed in mammalian cells, such as human cells, to produce, for example, polypeptides that are useful in developing novel anti-HIV antibodies. Polypeptides described herein and novel antibodies developed therefrom are generally useful for medical diagnostics, and they may also be useful in the prophylactic and therapeutic treatment of HIV.本發明之各個實施例係關於包含HIV外套膜蛋白及融合蛋白之1至10個或更多個表位之多肽，其中前述多肽缺少HIVgp41蛋白之跨膜結構域。前述多肽可在哺乳動物細胞例如人類細胞中表現，產生例如可用於研發新穎抗-HIV抗體之多肽。本文所述之多肽及自其研發之新穎抗體通常可用於醫學診斷，且其亦可用於HIV之預防及治療性治療。
