Federalnoe gosudarstvennoe avtonomnoe uchrezhdenie "Mezhotraslevoj nauchno-tekhnicheskij kompleks "Mikrokhirurgiya glaza" imeni akademika S.N. Fedorova" Ministerstva zdravookhraneniya Rossijskoj Feder
发明人:
Kostenev Sergej Vladimirovich (RU),Костенев Сергей Владимирович (RU),Malyugin Boris Eduardovich (RU),Малюгин Борис Эдуардович (RU),Mushkova Irina Alfredovna (RU),Мушкова Ирина Альфредовна (RU),Gerasim
申请号：
RU2018109504
公开号：
RU0002676451C1
申请日:
2018.03.19
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention relates to medicine, specifically to ophthalmology. To conduct corneal collagen crosslinking in the experiment, the cornea is treated with a solution of 0.1 % riboflavin for 30 minutes, the cornea is irradiated and its surface is wetted during the irradiation process with this solution every 2 minutes. Cornea is irradiated by a femtosecond laser with a wavelength of 525 nm. For this purpose, the human donor cornea is placed in an artificial anterior chamber, the central zone of the cornea is irradiated with a laser in the form of a straight circular cylinder 4 mm in diameter, 1 mm high, the upper base of which is the central zone of the cornea, and the longitudinal axis of the cylinder coincides with the optical axis of the eye. Set of identical layers is formed with a distance between them of 10 microns, the plane of which is perpendicular to the longitudinal axis of the cylinder. Each layer is formed from voxels located 2 mcm apart from each other in one row in height. Each voxel is an elliptical cylinder, the longitudinal axis of which lies in the plane of the layer, the major axis of the base perpendicular to the plane of the layer. In each layer, the longitudinal axes of the voxels of one layer are shifted by 90 degrees relative to the longitudinal axes of the voxels of the next layer.EFFECT: method increases the density of the cornea structure and its ability to resist stretching due to the cross-linking of collagen fibers.1 cl, 1 dwg, 1 exИзобретение относится к области медицины, а именно к офтальмологии. Для проведения кросслинкинга роговичного коллагена в эксперименте проводят обработку роговицы раствором 0,1% рибофлавина в течение 30 минут, облучение роговицы и смачивание ее поверхности в процессе облучения указанным раствором каждые 2 минуты. При этом облучение роговицы производят фемтосекундным лазером с длиной волны 525 нм. Для чего размещают донорскую роговицу человека в искусственной передней камере, лазером облуча
