Hadasit Medical Research Services and Development Ltd.
发明人:
Alper-Pinus, Ruslana,Banin, Eyal,Idelson, Masha,Reubinoff, Benjamin,Obolensky, Alex
申请号：
AU2008242106
公开号：
AU2008242106B2
申请日:
2008.04.27
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
The present invention concerns RPE cells obtainable by directed differentiationfrom stem cell, particularly, human stem cells. It has been specifically foundthat culturing stem cells in the presence of one or more member of the TGF superfamily,such as Activin A) induced directed differentiation into mature and functionalRPE cells. This was evidenced by the expression of markers specific to matureRPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particularembodiment, the cells are a priori cultured with nicotinamide (NA) which wasfound to augment the cells' response to the inductive effect of the one ormore member of the TGF superfamily. The invention also provides methods of performingthe directed differentiation, as well as methods for use of the resulting RPEcells.
