#$%^&*AU2020101473A420200903.pdf#####13 ABSTRACT The present invention belongs to the technical field of conditioners, and particularly relates to a rice conditioner for promoting rice seed germination and a preparation method and use thereof. The rice conditioner for promoting rice seed germination includes the following components in parts by mass: 1-4 parts of dimethyl sulfoxide, 1-4 parts of zinc sulfate, 0.01-0.04 parts of thidiazuron, 5-8 parts of glycine betaine, 0.5-2 parts of sterilant and 31.96-42.49 parts of palygorskite. Combination of thidiazuron, dimethyl sulfoxide, zinc sulfate, glycine betaine and sterilant in the conditioner of the present invention not only activates the activities of active substances and enzymes in rice seeds, protects against adverse environment and enhances freezing resistance of seedlings; but also improves germination force and root activity, promotes development of root systems, improves disease resistance of seedlings, and promotes healthy growth of seedlings.