THE SCRIPPS RESEARCH INSTITUTE;MAYFIELD, STEPHEN, P.;FRANKLIN, SCOTT
发明人:
MAYFIELD, STEPHEN, P.,FRANKLIN, SCOTT
申请号：
WO2003US12997
公开号：
WO03091413(A2)
申请日:
2003.04.23
申请国别(地区):
世界知识产权组织国际局
年份:
2003
代理人:
摘要：
Method of producing one or more polypeptides in a plant chloroplast, including methods of producing polypeptides that specifically associate in a plant chloroplast to generate a functional protein complex, are provided. An isolated polynucleotide that includes (or encodes) a first ribosome binding sequence (RBS) operatively linked to a second RBS, such that the first RBS directs translation of a polypeptide in a prokaryote and the second RBS directs translation of the polypeptide in a chloroplast, also is provided, as is a vector containing such a polynucleotide, particularly a chloroplast vector and a chloroplast/prokaryote shuttle vector. Also provided is a synthetic polynucleotide, which is chloroplast codon biased. A plant cell that is genetically modified to contain a polynucleotide or vector as described above, as well as transgenic plants containing or derived from such a genetically modified cell, are provided. Polypeptides encoded by a synthetic polynucleotide as described also are provided.
