In a method for the acquisition of optical coherence tomography image data of retina tissue of an eye (99) of a human subject using an acquisition device comprising an imaging optics (2), a first image associated with a baseline relative positioning of the eye (99) of the human subject with respect to the imaging optics (2) is acquired at a first point in time. The baseline relative positioning is stored. At a second point in time being different from the first point in time, the baseline relative positioning of the same eye (99) of the same human subject with respect to the imaging optics (2) is re-established and a second image is acquired. For re-establishing the positioning, a present relative positioning of the eye (99) of the human subject with respect to the imaging optics is determined based on a video image of an iris region of the eye. A corresponding device comprises an imaging optics (2), a head support to be contacted by a head portion of the human subject, the head support defining an entrance position of the sample beam entering an eye of the human subject, a camera (71 ) for acquiring a video image of an iris region of the eye, a display (75) for displaying a target image to the human subject, the target image indicating a direction of a line of vision to be assumed by the human subject, and a processor for determining a present relative positioning of the eye of the human subject with respect to the imaging optics, based on the video image, for comparing a present relative positioning of the eye (99) of the human subject with respect to the imaging optics (2) to a stored baseline relative positioning, for affecting the target image as long as the present relative positioning does not correspond to the stored baseline relative positioning, and for triggering the acquisition of image data when the present relative positioning corresponds to the baseline relative positioning.
