A sterilization device includes a base seat and a portable container. The base seat includes a base body, a circuit board, a light sensor electrically connected to the circuit board, and a UV light generator electrically connected to the circuit board. The portable container is detachably disposed on the base body and includes a hollow body and a light-transmissive board. The hollow body and the light-transmissive board cooperatively surround a containing space therebetween. When the portable container is mounted on the base body, the light sensor is covered by the portable container without receiving an environmental light, thus the UV light generator is driven by the circuit board to generate an UV light for sterilization of the containing space. When the portable container is removed from the base body, the light sensor receives the environmental light, so the circuit board stops the sterilization of the UV light generator.一種殺菌裝置包括殺菌基座及可攜式容器。殺菌基座包括基座本體、電路板、電性連接電路板的光感測器及紫外線光產生器。可攜式容器可拆卸地安裝於基座本體，並包括中空本體與配置於中空本體的透光底板，中空本體與透光底板共同環繞出容納空間。當可攜式容器安裝於基座本體上時，光感測器被可攜式容器覆蓋而接收不到外界光線，電路板控制紫外線光產生器作動而朝透光底板發出紫外線光，紫外線光穿透透光底板對容納空間殺菌。當可攜式容器移離基座本體時，光感測器接收到外界光線，而使電路板控制紫外線光產生器停止作動。
