MYERS KENT R.,MYERS Kent R.,SNYDER D. SCOTT,SNYDER D. Scott
申请号：
SI200231029
公开号：
SI1461418T1
申请日:
2002.03.28
申请国别(地区):
SI
年份:
2013
代理人:
摘要：
Herein is disclosed a method for producing lipopolysaccharide (LPS), comprising: (a) growing a culture of a deep rough mutant bacterial strain in a medium (b) maintaining the culture in stationary phase for at least about 5 hr (c) harvesting cells from the culture and (d) extracting LPS from the cells. The method allows for the production of an LPS which can be used to produce a 3-0-deacylated monophosphoryl lipid A (3D-MLA) having at least about 20 mol% of the hexaacyl congener group. Also herein is disclosed a method of extracting lipopolysaccharide (LPS) from a culture of deep rough mutant bacterial strain cells, comprising: (a) extracting the cells with a solution consisting essentially of at least about 75 wt% of an aliphatic alcohol having from 1 to 4 carbon atoms and the balance water, thereby producing cells with reduced phospholipid content and (b) extracting the cells with reduced phospholipid content with a solution comprising chloroform and methanol, thereby yielding a solution of LPS in chloroform and methanol (mM). This method provides LPS solutions in CM that have reduced phospholipid content and are produced by relatively simple and inexpensive process steps.
