PROCÉDÉ PERMETTANT DE PRODUIRE EN MASSE UNE CELLULE TUEUSE NATURELLE ET UTILISATION DE LA CELLULE TUEUSES NATURELLE OBTENUE PAR LEDIT PROCÉDÉ EN TANT QU'AGENT ANTI-CANCÉREUX
Korea Research Institute of Bioscience and Biotechnology
发明人:
申请号：
EP16743625.2
公开号：
EP3252152A1
申请日:
2016.01.15
申请国别(地区):
EP
年份:
2017
代理人:
摘要：
The present invention relates to a method for producing a large amount of natural killer cells and the use of natural killer cells obtained by the method as an anticancer agent. The use of the method of the present invention can produce fresh NK cells with high purity within a short time compared to conventional method, and can also produce cold-preserved NK cells and thawed cryopreserved NK cells, which have efficacy comparable with that of the fresh NK cells. Furthermore, it can produce NK cells, which have efficacy comparable with that of the fresh NK cells, from cryopreserved CD3-negative cells. The fresh NK cells, cold-preserved NK cells and cryopreserved NK cells produced by the methods of the present invention can exhibit therapeutic effects against various cancers, including colorectal cancer, lung cancer, liver cancer, pancreatic cancer and leukemia, indicating that these NK cells can be effectively used as cellular therapeutic agents. In addition, the present inventors have established doses and methods of administration, which show excellent effects when the fresh NK cells, cold-preserved NK cells and cryopreserved NK cells of the present invention are used as pharmaceutical compositions for cellular therapy.
