INSTITUTE OF ANIMAL SCIENCES, CHINESE ACADEMY OF AGRIGULTURAL SCIENCES
发明人:
Li Kui,Ruan Jinxue,Yang Shulin,Mu Yulian,Li Hegang,Wu Tianwen,Wei Jingliang,Xu Kui,Huang Lei,Zhou Rong,Liu Nan
申请号：
US201415531717
公开号：
US2018105834(A1)
申请日:
2014.11.27
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
The present invention provides a method of site-directed insertion to H11 locus in pigs by using site-directed cutting system, includes the following steps: 1) identify the targeted sequence targeted by the targeted cutting system in the targeted genome sequence of pigs; 2) design and construct the targeting sequence of the corresponding cutting system according to the targeted site; 3) construction of targeting vector; 4) transfect cells, identify the efficiency of fixed-point insertion by PCR amplification. The invention is dependent on the site-directed cutting system of H11 locus in pigs, to insert the target gene into the target site, in order to solve the problems such as low efficiency of traditional shooting technique, inconvenience design of PCR detection primer, harder to detect. The invention provides a method of site-directed insertion which can stably express the foreign gene at the H11 locus, to build an efficient platform for the production of transgenic pigs.
