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DISCRIMINATION OF PLANT CONTAINING CYTOPLASMIC MALE STERILITY FACTOR DNA AND CYTOPLASMIC MALE STERILITY FACTOR DNA TO BE USED THEREFOR
专利权人:
SUMITOMO CHEM CO LTD
发明人:
NAKAJIMA ARINORI,YAMAMOTO TOSHIYA,OITA KENJI
申请号:
JP19960243201
公开号:
JPH1084998(A)
申请日:
1996.09.13
申请国别(地区):
日本
年份:
1998
代理人:
摘要:
PROBLEM TO BE SOLVED: To easily discriminate the sterile character of a plant by using a cytoplasmic male sterility factor DNA having a specific base sequence as a primer, amplifying a plant genom DNA by PCR method, separating by electrophoresis and visually detecting the objective DNA. SOLUTION: A genom DNA of a plant (e.g. carrot) is amplified by PCR method using an oligonucleotide effective for amplifying cytoplasmic male sterility factor DNA having a base sequence expressed by the formula, a part of the DNA, a DNA containing the DNA or a compound having the similar action as a primer. The amplified genom DNA is separated by electrophoresis and visually detected to enable the easy and quick confirmation of the genetic state of cytoplasmic male sterility character without necessitating much labor and long time. A plant having cytoplasmic male sterility factor DNA useful for the development of the breeding to control the fertility of a plant can be discriminated by this process.
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中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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