The present invention relates to a hair loss-preventing and hair growth-promoting composition, a producing method thereof, and use thereof, the composition containing, as an active ingredient, a culture medium of stem cells stimulated by a certain catagen inducer containing TGF-β, and the use of the composition being the use of the function of very effectively growing hair by means of the catagen inducer stimulating the stem cells, thereby enabling the secretion of Wnt3a, Bcl-2, CyclinD-1, etc. which are known to be proteins sending signals related to hair tissue differentiation.La présente invention concerne une composition prévenant la chute des cheveux et favorisant la croissance des cheveux, son procédé de production associé, et son utilisation, ladite composition contenant, en tant que principe actif, un milieu de culture de cellules souches stimulées par un certain inducteur catagène contenant le TGF-beta, et lutilisation de ladite composition étant lutilisation de la fonction permettant la croissance très efficace des cheveux au moyen de linducteur catagène stimulant les cellules souches, permettant ainsi la sécrétion de Wnt3a, Bcl-2, CyclineD-1, etc. qui sont connus pour être des protéines envoyant des signaux associés à la différenciation tissulaire des cheveux.본 발명은 TGF-β를 포함하는 특정 모발 퇴행기 유도인자(catagen inducer)로 자극된 줄기세포 배양액을 유효성분으로 함유하는 탈모 방지 및 발모 촉진용 조성물, 이의 제조 방법 및 용도에 관한 것으로, 상기 모발 퇴행기 유도인자가 줄기세포를 자극하여 모발조직 분화 관련 신호전달 단백질로 알려진 Wnt3a, Bcl-2, CyclinD-1 등을 분비시킴으로써 매우 효과적으로 헤어를 성장시키는 기능을 이용하는 용도에 관한 것이다.