LIU, JE RUEI,刘嚞睿,劉嚞睿,YI, PING JUNG,易秉蓉,易秉蓉,PAI, CHENG KANG,白正康,白正康
申请号：
TW099100613
公开号：
TWI418303B
申请日:
2010.01.11
申请国别(地区):
TW
年份:
2013
代理人:
摘要：
The present invention discloses an isolated pure culture of a novel strain (CK1) ofBacillus licheniformis, BCRC 910458, capable of detoxification of zearalenone (ZEN). Using physiological, biochemical, morphological identification and 16S rRNA gene sequence analysis methods, CK1 was identified asBacillus licheniformis. Through extracellular xylanase, CMCase protease activity assays and evaluations for zearalenone detoxification, CK1 strain was identified to be non-hemolytic, non-enterotoxin producing, and displayed high levels of extracellular xylanase, cellulase, and protease activities. Accordingly,B. licheniformisCK1 has the potential for the bio-detoxification of ZEN in food and feed.本發明揭露一種由土壤樣本所分離出之地衣芽孢桿菌CK1菌株(BCRC 910458),經過生理、生化、型態學及基因特徵的鑑定,證實CK1菌株確實屬於地衣芽孢桿菌。經由評估其細胞外聚木糖酶(xylanase)、羧甲基纖維素酶(CMCase)、蛋白酶(protease)活性,以及經由對於CK1菌株分解玉米烯酮毒素(zearalenone)之各種檢測,證明本發明之地衣芽孢桿菌CK1菌株具有良好的玉米烯酮毒素解毒能力、不具溶血性、不會產生腸毒素,適合用於添加於受到玉米烯酮毒素污染的食物或飼料,去除玉米烯酮毒素的危害。
