PROBLEM TO BE SOLVED: To provide an artificial lipid membrane forming method, for producing an artificial lipid membrane chamber having an artificial lipid membrane that can be directly observed using electrodes and a microscope, which is simple and provides high throughput, and to provide an artificial lipid membrane forming apparatus suitable for applying such method.SOLUTION: An artificial lipid membrane forming method uses an artificial lipid membrane forming apparatus. The apparatus includes a substrate having a hydrophobic layer and hydrophilic patterns formed thereon and a solution injection unit for forming droplets on the hydrophilic patterns on the substrate. The method includes: a first solution injection step for injecting water or water solution onto the substrate and forming droplets on the hydrophilic patterns; a lipid solution injection step for injecting a lipid solution onto the substrate before or after the first solution injection step; and a second solution injection step for injecting wa
