The present invention relates to isolated peptide from pneumolysin (PLY) and their antagonists 4 (TLR-4) as Toll-like receptor. The TLR-4 that the combination of the peptide causes activity to decline. Particularly, the present invention provides isolated peptides carries out under the conditions of effectively treating TLR-4 related activations from the end regions C- short for plywood, such as diabetes and atherosclerosis.