Provided are a universal T cell and a preparation method therefor. The universal T cells comprise CAR-T and TCR-T cells, and are obtained by knocking out TCR and/or HLA and/or PD-1 proteins of T cells using CRISPR/Cas9 gene editing technology, wherein the universal CAR-T can continue to kill target cells in vivo and in vitro.本發明涉及一種新型的通用型T細胞（包括CAR-T和TCR-T細胞）及其製備方法。在一些實施方式中，該通用T細胞通過CRISPR/Cas9基因編輯技術，同時高效、穩定地敲除了T細胞的TCR和/或HLA和/或PD-1蛋白。本發明的通用型CAR-T在體內、體外具有高效持續的殺傷靶細胞的作用。
