ГАНШАНИ Санджив (US),ЛЕ Линх К. (US),ЛЮ И (US),СТЮАРД Лэнс Э. (US)
申请号：
RU2012129557/10
公开号：
RU2012129557A
申请日:
2010.12.14
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A single-chain modified Clostridial toxin containing: a) the enzyme domain of the Clostridial toxin capable of performing the enzyme directed modification stage of the Clostridial toxin process； b) the translocation domain of the Clostridial toxin capable of performing the translocation stage of the Clostridial toxin process； and c) an integrated protease cleavage site-binding domain containing the P portion of the protease cleavage site, including the P site of the unstable chemical bond and a binding domain, the P site of the protease cleavage site adjacent to the amino terminus of the binding domain, thereby creating an integrated protease cleavage site； where cleavage of the integrated site-binding domain of the protease cleavage converts the single-chain modified Clostridial toxin into a double-stranded form and forms a binding domain with an amino end capable of binding to its cognate receptor. 2. The modified Clostridial toxin according to claim 1, characterized in that the modified Clostridial toxin contains a linear amino carboxyl single polypeptide order of 1) the enzyme domain of the Clostridial toxin, the translocation domain of the Clostridial toxin and the integrated site-binding domain of the protease cleavage, 2) the enzyme domain of the Clostridial toxin integrated site-binding domain of protease cleavage and translocation domain of the toxin Clostridial, 3) integrated site-binding domain of protease cleavage, translocation the domain of the Clostridial toxin and the enzyme domain of the Clostridial toxin, 4) an integrated site-binding domain of protease cleavage, the current enzyme domain1. Одноцепочечный модифицированный токсин Clostridial, содержащий:a) ферментный домен токсина Clostridial, способный выполнять стадию ферментной направленной модификации процесса интоксикации токсина Clostridial；b) транслокационный домен токсина Clostridial, способный выполнять стадию транслокации процесса интоксикации токсина Clostridial； иc) интегрированный са
