A method of hemostasis includes the step of applying a hemostatic agent to an affected site of a subject, the hemostatic agent consisting of a first agent comprising a gelatin derivative having a hydrophobic group bonded to the gelatin via an imino group, wherein the gelatin derivative has (a) a weight average molecular weight of from 10,000 to 50,000, (b) the hydrophobic group, which is an alkyl group having 6 to 18 carbon atoms； and (c) a molar ratio of imino group/amino group of the gelatin derivative ranging from 1/99 to 30/70； and a second agent including a crosslinking agent for the gelatin derivative.
