A fatty acid composition containing linoleic acid, linolenic acid, and oleic acid is provided. Also provided is a fatty acid composition containing linoleic acid, linolenic acid, and oleic acid, and at least one selected from palmitinic acid, palmitoleic acid, stearic acid, arachidic acid, and docosanoic acid. A plant extract and a pharmaceutical preparation are provided, wherein the pharmaceutical preparation contains an active component including at least one of the fatty acid compositions, the plant extract and modified products thereof. Also provided is an application of the fatty acid composition, the plant extract and the pharmaceutical preparation in multiple fields. The pharmaceutical preparation may function to repair various wounds and traumas in skin, mucosa, lumina and muscular tissues.