The present invention relates to a bio-sensing device capable of detecting sensing code and short fill, the bio-sensing device comprises: a bio-sensing strip and a process-and-display unit, the bio-sensing strip has a first recognition membrane, a second recognition membrane, a third recognition membrane, and a sensing code resistor, wherein the three recognition membranes is able to execute electrochemical reaction with a sample, and the sensing code resistor represents a strip code of the bio-sensing strip the process-and-display unit comprises: a first read circuit and a second read circuit, wherein the first read circuit is used to read the value of the sensing code resistor so as to facilitate the process-and-display unit obtaining the strip code automatically, besides, the first read circuit can detect whether the sample covers the first recognition membrane and second recognition membrane or not for confirming the quantity of the sample injected into the bio-sensing strip is enough, so that the process-and-display unit calculates an accurate sample magnitude value.本發明係關於一種可偵測試片碼與檢體填充不足之生物感測裝置,其包括一生物感測試片與一處理顯示單元,該生物感測試片之上係設有一第一辨識膜、一第二辨識膜、一第三辨識膜、與一感測碼電阻,該三個辨識膜可與一檢體進行電化學反應,該感測碼電阻之電阻值可表示為生物感測試片之一試片碼;該處理顯示單元具有一第一讀取電路與一第二讀取電路,其中,該第一讀取電路可讀取感測碼電阻之電阻值,使得處理顯示單元可自動獲得該試片碼,並且,第一讀取電路可偵測檢體是否已經完全覆蓋第一辨識膜與第二辨識膜,以確認注入生物感測試片之檢體為足量,使得處理顯示單元可準確地計算出檢體之一檢體量值。11...生物感測試片111...第一電極1111...第一辨識膜1112...第二辨識膜1113...第三辨識膜112...第二電極113...第三電極114...檢體流道115...虹吸氣孔121...第一讀取電路1211...第一運算放大器1212...第一類比/數位轉換器122...第二讀取電路1221...第二運算放大器1222...第二類比/數位轉換器R1...第一電阻R2...第二電阻Rs...感測碼電阻Vref...參考電壓