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A method of activating stem cell proliferation and improving stem cell resistance to negative effects.
专利权人:
オルロヴァ エレナ ウラディミロヴナ;マエフスキー エフゲニー イリチ;クラブコフ ウラディミール コンスタンチノヴィッチ;ゼリスコ アレクセイ パブロヴィッチ;オルロヴァ エレナ ウラディミロヴナ
发明人:
オルロヴァ エレナ ウラディミロヴナ,マエフスキー エフゲニー イリチ,クラブコフ ウラディミール コンスタンチノヴィッチ
申请号:
JP2015534426
公开号:
JP2015532092A
申请日:
2012.10.02
申请国别(地区):
JP
年份:
2015
代理人:
摘要:
The present invention relates to the fields of biotechnology, medicine, pharmacology, cell biology, and biotechnology, and utilizes stem cells and progenitor cells of various stages of differentiation and mature cells after mitosis of various tissues. It can also be used in other areas of biology and medicine, particularly for rapid development of biological grafts from human and / or animal donors or autoimmune stem cells. The technical result aimed at by the present invention is activation of stem cell proliferation and improved tolerance to the negative effects of human and animal stem cells, independent of the negative effects of high frequency electromagnetic fields. The technical result is achieved by treating the cell culture with a weak low frequency magnetic field. The proposed method is characterized by the following: 1). When human stem cells are cultured after exposure to a weak magnetic field, typically for half a period of doubling the culture of a given cell, eg 24 hours, the number of cells increases and the increase in cell number is 2.5 times. (With 48 hours of culture equal to the total long-term doubling of such cells). 2). Increasing the amplitude of self-magnetic irradiation of primary cultures of stem cells as measured with a SQUID magnetometer, indicating an increase in culture activity. 3. When human stem cells are exposed to a weak magnetic field, typically half the period of doubling of a given cell culture, they stabilize twice in terms of apoptosis and are primarily synchronized in the G1 phase of the cell cycle. To do. 3). When human stem cells are exposed to a weak magnetic field, typically half the period of doubling of a given cell culture, they are twice as stable in terms of apoptotic expression and are primarily G1 in the cell cycle. Tune in phase. [Selection figure] None本発明は、バイオテクノロジー、医薬、薬理学、細胞生物学、および生物工学の分野に関するものであり、様々な分化段階の幹細胞および前駆細胞ならびに様々な組織の有糸分裂後の成熟細胞を利用する生物学および医薬の他部門においても利用可能であり、特にヒトおよび/または動物のドナーまたは自己免疫性幹細胞から生体移植片を急速発達させるために
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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