PROCEDE DE SELECTION ET DE PRODUCTION DE MOLECULES THERAPEUTIQUES MULTI-SPECIFIQUES, SELECTIVES ET PERSONNALISEES COMPRENANT AU MOINS DEUX ENTITES DE CIBLAGE DIFFERENTES ET LEURSUTILISATIONS
HEINDL, DIETER,HUELSMANN, PETER MICHAEL,KALUZA, BRIGITTE,KOPETZKI, ERHARD,NIEDERFELLNER, GERHARD,TIEFENTHALER, GEORG
申请号：
CA2871386
公开号：
CA2871386A1
申请日:
2013.06.25
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
Herein is reported a method for determining a combination of antigen binding sites comprising the steps of (i) determining the binding specificity and/or affinity and/or effector function and/or in vivo half-life of a multitude of bispecific antibodies prepared by combining each member of a first multitude of antibody Fab fragments or scFv antibody fragments with each member of a second multitude of antibody Fab fragments or scFv antibody fragments, and a linker comprising at one of its termini the second member of the first binding pair and at the respective other terminus the second member of the second binding pair, whereby the first multitude specifically binds to a first cell surface molecule and the second multitude specifically binds to a second cell surface molecule, and (ii) choosing the bispecific antibody with suitable binding specificity and/or affinity and/or effector function and/or in vivo half-life and thereby determining a combination of antigen binding sites.
