Chung Hang Jonathan Choi,Zhong Chen,Lei Zhang,Huize Li
申请号：
US15708810
公开号：
US10533189B2
申请日:
2017.09.19
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The subject invention pertains to delivering a polynucleotide into a target cell, particularly, into the nucleus of the target cell. The method comprises the steps of contacting the polynucleotide with the cell and applying pressure on the polynucleotide and the cell in a manner that forces the polynucleotide into the cell. The pressure can be between about 0.1 Pa to about 50 Pa； whereas, the polynucleotide is contacted with the cell at a concentration of between about 0.1 μM and about 100 μM. The method can be practiced for cells in culture or cells in vivo.
