A living body observation system includes: an illuminating light generation section configured to generate first light belonging to a blue region and second light belonging to a red region, as light for illuminating an object provided with a pigment with an absorption peak in the red region an image pickup section a computation section configured to calculate a difference degree indicating a degree of difference between a luminance value of a first image obtained by picking up an image of return light of the first light and a luminance value of a second image obtained by picking up an image of return light of the second light in a same pixel an enhancement coefficient calculation section configured to calculate an enhancement coefficient for a pixel in which the difference degree exceeds a predetermined threshold an enhancement processing section configured to enhance a contrast of a pixel in which the difference degree exceeds a predetermined threshold among respective pixels included in the first image based on the enhancement coefficient and an observation image creation section configured to create an observation image by using the first image after the process by the enhancement processing section and the second image.
