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Food additive composition
专利权人:
DuPont Nutrition Biosciences ApS
发明人:
MILLAN, Luis Fernando Romero
申请号:
ES12701263
公开号:
ES2657543T3
申请日:
2012.01.19
申请国别(地区):
ES
年份:
2018
代理人:
摘要:
A food additive composition comprising a direct feed microorganism (DFM) in combination with a subtilisin and a 6-phytase, in which the direct feed microorganism is a direct feed antipathogen microorganism, in which the direct feed microorganism it is an antipathogen when it produces a reduced optical density (OD) compared to a control in the following DFM test: i) tubes are seeded with a pathogen representative of a representative group; ii) supernatant from a potential aerobically or anaerobically grown DFM is added to the seeded tubes and incubated; and iii) after incubation, the OD of the tubes treated with control and supernatant for each pathogen is measured, in addition to which the DFM is present in a dose between 3.75 x 107 CFU / g of food additive composition and 1 x 1011 CFU / g of food additive composition, subtilisin is present in a dose between 1,000 UP / g of food additive composition and 60,000 UP / g of food additive composition, and 6-phytase is present in a dose between 200 FTU / g of food additive composition and 40,000 FTU / g of food additive composition, and in which DFM comprises a bacterium of one or more of the following species: Bacillus subtilis, Bacillus licheniformis and combinations thereof.Una composición de aditivo alimentario que comprende un microorganismo de alimentación directa (DFM) en combinación con una subtilisina y una 6-fitasa, en el que el microorganismo de alimentación directa es un microorganismo de alimentación directa antipatógeno, en el que el microorganismo de alimentación directa es un antipatógeno cuando produce una densidad óptica reducida (DO) comparado con un control en el siguiente ensayo DFM: i) se siembran tubos con un agente patógeno representativo de un grupo representativo; ii) se agrega sobrenadante de un DFM potencial cultivado aeróbicamente o anaeróbicamente a los tubos sembrados y se incuba; y iii) después de la incubación, se mide la DO de los tubos tratados con control y sobrenadante para cada patóge
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