PROBLEM TO BE SOLVED: To provide a plant cultivation device in which a cultivation tray and a plant stock can be efficiently carried in or out at an optimal timing, useless space in an early stage of growth is reduced, an amount of cultivation per unit space is made to increase, and space efficiency is improved, and working efficiency is raised, and thereby which can provide an optimal environment according to growth.SOLUTION: The plant cultivation device 100 can grow two or more plant stocks S which are arranged at two or more steps and two or more sequences, wherein a specific cultivation tray hold shelf 110 of two or more cultivation tray hold shelves 110 is configured as an operation station 130 in which watering operation required for cultivation is possible, and which combines input/output parts taking in/taking out a cultivation tray 120 or the plant stock from an external/to the external.