The present invention is directed to direct-fed microbials for use in improving the performance of an animal, improving the health of an animal, improving the environment of an animal, and combinations thereof. More particularly, the invention relates to isolated Bacillus strains 86 and 300, and strains having all of the identifying characteristics of these strains, for uses comprising the above-mentioned uses.本發明係關於用於改善動物效能、改善動物健康、改善動物環境及其組合之直接餵飼之微生物。更特定言之,本發明係關於分離芽孢桿菌菌株86及300,及具有此等菌株之所有鑑別特徵之菌株,其用於包含上文所提及之用途在內的用途。