The present invention aims to provide a method of producing motor neurons/neurons that sufficiently reproduce intrinsic properties of motor neurons/neurons, especially of the motor neurons/neurons in patients, from pluripotent stem cells promptly and synchronically, and a pluripotent stem cell capable of differentiating into a neuron or a motor neuron promptly and synchronically after a drug treatment.A method of generating a motor neuron from a pluripotent stem cell, comprising the following steps in order from (1) to (2):(1) introducing one or more nucleic acids encoding Lhx3, Ngn2, and Isl1 into a pluripotent stem cell； and(2) maintaining expression of the Lhx3, Ngn2, and Isl1 for three days or more.
