A population of human stem cells is, or is selected to be, positive for CD271 and acetylated tubulin. Cells are isolated by a method comprising isolation of a cell from a mixed population of cells based on expression of cell surface markers, wherein the markers are CD271 and acetylated tubulin. The isolated cells may be used in therapy for example by producing tissues such as bone, cartilage or tendon, or in pharmaceutical compositions comprising isolated cells.
