Disclosed is an AGR2 blocking monoclonal antibody, and in particular, a humanized monoclonal antibody for blocking AGR2. Also disclosed is a pharmaceutical composition containing the antibody and a method for preparing the same, and a use of the antibody in blocking tumor growth and metastasis.本發明涉及一種AGR2阻斷性單株抗體,尤其是阻斷AGR2的人源化單株抗體。本發明還涉及包含該抗體的藥物組合物,製備方法及用途。該抗體可用於阻斷腫瘤生長和轉移。