The present invention relates to the methods for making pluripotent stem cell differentiation. In particular, the present invention provides the method characterized using cell of the unique surface marker to the cell for being divided into expression pancreatic endocrine pedigree characteristic markers. The present invention also provides the methods that the cell to expression pancreatic endocrine pedigree characteristic markers is enriched with or sorts. The present invention also provides the cells to the cell mass that may pollute the expression pancreatic endocrine pedigree characteristic markers formed by the method for the present invention to be purged, to reduce the method that in-vivo tumour after transplanting forms incidence.
