The present invention describes methods and compositions of hydrogel microspheres for inducing macrophage conversion by sequentially converting a first population of wound macrophages in a wound to M2A macrophages by exposing the first population of wound macrophages to IL-4 and converting a second population of wound macrophages to M2C macrophages by exposing the second population of wound macrophages to IL-10. One aspect describes a method of inducing macrophage conversion in a wound. Another aspect describes compositions and methods of preparing a hydrogel microsphere for wound healing. Yet another aspect describes methods for treating a chronic wound with hydrogel microspheres.
