The present invention relates to a method using connective tissue for preparation of collagen wound dressing. First, a high-purity collagen is extracted from connective tissue of terrestrial or aquatic animals then, the high-purity collagen is utilized for preparation of collagen wound dressing, wherein the collagen wound dressing has the functions of facilitating hemostasis, wound healing, and tissue repair and is suitable for the treatment of small wounds or surgical wounds.本發明係為一種以結締組織製備高純度膠原蛋白傷口敷料的方法,其主要是以陸生動物或水生動物之結締組織來萃取一高純度膠原蛋白後,再以該高純度膠原蛋白來製備膠原蛋白傷口敷料;其中該膠原蛋白傷口敷料具有促進止血、傷口癒合及修復組織等作用,適用於一般傷口或手術創傷處理。S201‧‧‧高純度膠原蛋白S202‧‧‧製備敷料均質液S203‧‧‧製備敷料片S204‧‧‧敷料片凍乾IS205‧‧‧敷料片覆水S206‧‧‧敷料片清洗S207‧‧‧敷料片凍乾II