BUNNIK, EVELIEN M,CUSTERS, JEROME H H V,SCHEPER, GERRIT CH,OOSTERHUIS, KOEN,UIL, TACO GILLES,KHAN, SELINA
申请号：
CA2965562
公开号：
CA2965562A1
申请日:
2015.11.03
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The invention provides designer nucleic acid constructs and polypeptides that can be used as therapeutic vaccines against HPV 16. These polypeptides comprise essentially all possible T-cell epitopes of HPV 16 oncoproteins E6 and E7, but nevertheless have a strongly reduced (as compared to wt E6 and E7), up to non-detectable, transforming activity, by comprising fragments of the E6 and E7 proteins that have been re-ordered, while at the same time containing a minimized number of undesired neo-epitopes. The invention provides a nucleic acid molecule encoding a polypeptide comprising a sequence as set forth in SEQ ID NO: 1. In certain embodiments, the encoded polypeptide further comprises at least one epitope of a human papillomavirus (HPV) E2 protein, for example an HPV 16 E2 protein.
