Johnson, Donald,Kernag, Casey A.,Bieniarz, Christopher,Kosmeder, Jerry W.
申请号：
AU2008262489
公开号：
AU2008262489B2
申请日:
2008.05.22
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
Certain disclosed embodiments of the present invention concern the synthesis,derivatization, conjugation to immunoglobulins and signal amplificationbased on discrete, relatively short polymers having plural reactive functionalgroups that react with plural molecules of interest. Reactive functional groups,such as hydrazides, may be derivatized with a variety of detectable labels, particularlyhaptens. The remaining reactive functional groups may be conjugated directlyto a specific binding molecule, such as to the oxidized carbohydrate of the Fcregion of the antibody. Disclosed conjugates display large signal amplificationas compared to those based on molecules derivatized with single haptens, andare useful for assay methods, particularly multiplexed assays.
