LING, Vincent,NYSTUEN, Ame,TAO, Weng,STABILA, Paul,KAUPER, Konrad
申请号：
ES11844999
公开号：
ES2613302T3
申请日:
2011.11.30
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
A cell line comprising: a. a genetically modified ARPE-19 cell that produces a therapeutically effective amount of one or more anti-angiogenic antibody structures or anti-angiogenic molecules, wherein the one or more anti-angiogenic antibody structures or anti-angiogenic molecules are introduced into the ARPE-19 cell for at least one transfection, in which the transfection comprises a transfection, two transfections, or three transfections, and wherein the one or more anti-angiogenic antibody structures or anti-angiogenic molecules are encoded by a sequence of nucleic acid selected from the group consisting of SEQ ID NO: 1 or has an amino acid sequence selected from the group consisting of SEQ ID NO: 2； or b. a genetically engineered ARPE-19 cell that produces a therapeutically effective amount of one or more structures of anti-angiogenic antibodies or anti-angiogenic molecules, wherein the therapeutically effective amount is at least 10,000 ng / day / 106 cells and wherein the one or more anti-angiogenic structures or anti-angiogenic molecules are encoded by a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, or has an amino acid sequence selected from the group consisting of SEQ ID NO: 2 .Una línea celular que comprende: a. una célula ARPE- 19 genéticamente modificada que produce una cantidad terapéuticamente eficaz de una o más estructuras de anticuerpos anti-angiogénicas o moléculas anti-angiogénicas, en donde las una o más estructuras de anticuerpos anti-angiogénicas o moléculas anti-angiogénicas se introducen en la célula ARPE-19 por al menos una transfección, en el que la transfección comprende una transfección, dos transfecciones, o tres transfecciones, y en donde la una o más estructuras de anticuerpos anti-angiogénicas o moléculas anti-angiogénicas se codifican por una secuencia de ácido nucleico seleccionada del grupo que consiste en SEQ ID NO: 1 o tiene una secuencia de aminoácido seleccionada del grupo que consiste en SEQ ID NO:2
