The present invention relates to a process for the large scale production of arbuscular mycorrhizal fungi inoculum with high infective propagule density by using aeroponic culture in twelve weeks (including nursery preparation). The invention specifically provides a nutrient composition which significantly enhances the growth of a plant in an aeroponic culture.The said process is a cost effective process for the mass production of arbuscular mycorrhizal fungi inoculums in short span of twelve weeks (including nursery preparation)