The systems and applications for generating augmented reality (AR) images are disclosed. The system includes a processing module and a digital microscope module having a pluralty of camera unit, and the processing module tracks, detects and parses the user’s motions, commands or event triggered to generate the related control signals, the virtual objects and then the composed AR images according to the received instant images of the observed samples captured by the digital microscope module. Moreover, the processing module generates and outputs the AR images composing of the instant images and the user interface(UI), icons, objects, video and/or informations related to the interactive applications while the display mode switch or the real-time tutorial and sharing is triggered.本發明揭露一種擴增實境(AR)影像產生系統及其應用，所述系統包含處理模組及數位顯微模組。數位顯微模組具有多個攝影單元，處理模組則用以追蹤、偵測並解析使用者操控動作以產生控制訊號，以及接收、處理數位顯微模組所擷取之包含對象物之瞬間影像，以產生虛擬物件並據以產生擴增實境影像。若使用者觸發例如對於對象物之顯示模式切換或即時導引或分享之開啟或關閉之互動應用，處理模組更用以產生疊合、顯示瞬間影像與所述互動應用或對象物相關聯之介面、圖像、物件、影片及/或資訊之擴增實境影像，以供該使用者繼續操作。
