<；P>；PROBLEM TO BE SOLVED: To provide a screening method and a proper evaluation system for developing a drug having cell-death inducing activity or proliferation suppressing activity on cancer cells or developing an assistant drug for recovering sensitivity of known anticancer agent resistant strains. <；P>；SOLUTION: Cancer cells are seminated at a low density and cultured to form a colony. A test drug is added, and a cell image is captured with time and compared with a result attained without adding the test drug to calculate the proliferation rate of the cell and cell-death inducing rate of the test drug on each colony. The average values of the proliferation rate of the cell and the cell-death inducing rate of the whole cell mass are calculated from the values of each colony together with variation coefficient of both data for each cell, and these values are calibrated by a control to evaluate the efficacy as an anticancer agent or as an assistant drug of known anticancer agents. Influence of the drug on normal cells is evaluated by performing contrast experiment on normal cells in the screening of the anticancer agent and on a system added with only the assistant drug in the case of screening the assistant drug. <；P>；COPYRIGHT: (C)2011,JPO&INPIT
